Supplementary information for
Improved Comparison of Protein Subcellular Location Patterns,
Ting Zhao, Stalia Soto and Robert F. Murphy,
Proceedings of the 2006 IEEE International Symposium on Biomedical Imaging (ISBI 2006), pp. 562-565.

A PDF file containing the paper can be downloaded from the Murphy lab publications page. The section numbers below refer to those in the paper.

The data and software used for the paper can also be downloaded.

• 2DHeLa Dataset (section 2.1)
To view the images in 2DHeLa dataset, follow the instructions below:
1. Go to http://murphylab.web.cmu.edu/services/PSLID/ and click on the link for "text and image content search interface";
2. Click Public Access at the bottom of the page. You will see a page with the title Murphy Lab PSLID Service;
3. Click the button Sets on the left side of the page;
4. Select "2D region sets" (the default) and click Continue. You will see the list of services:
   TypIC Relevance FB SImEC SLIC Clustering Feature Set Management
5. Click Set Management. You will see the Set Management portion of the Sets page;
6. Select a set under Set Management;
   The sets for the 2DHeLa dataset are:
   

   Set Name	Pattern
   2DHeLaGpp	gpp130
   2DHeLaDNA	DAPI
   2DHeLaLys	LAMP2
   2DHeLaNucle	nucleolin
   2DHelaActin	actin
   2DHelaER	ER
   2DHelaGia	giantin
   2DHelaTubul	tubulin
   2DHelaMito	mc151
   2DHelaTfR	tfr

7. Click View Selected Set button. You will see the list of images in the selected set. The images in this page are thumbnails.
8. To view an image of original size, click the link right under its thumbnail. A new browser window will pop up. There is a table in the new window. Click Original image in the 1st row and the 2nd column of the table. Then you will see the larger image.
• 3D3T3 Dataset (section 2.2)
The images in the 3D3T3 dataset can be viewed either in PSLID as described above but from within the PSLID Subcellular Location Tree browser at http://murphylab.web.cmu.edu/services/PSLID/tree.html.
• 3D3T3 Drug Dataset (section 2.3)
The images in the 3D3T3 Drug dataset can be viewed in PSLID as described above for the 2DHeLa dataset, with the following modifications: Select "3D region set" in step 4 and select one of the 3D3T3 Drug sets in step 6.
• Examples of feature histograms (section 4)
Each of the following figures shows the histograms of one feature for the images of giantin and gpp130 in 2DHeLa dataset. The X axis is the feature value. (Fig. 1-3 show features that are found to be different by the BH method. Fig. 4 shows a feature that shows no difference.)