-Image Specifications- The 3D 3T3 collection was collected in collaboration with Dr. Jonathan Jarvik and Peter Berget and consists of fluorescence microscope images of cell lines expressing GFP-tagged proteins. The cell lines were obtained by CD-tagging to produce internal GFP-fusions in random proteins. Cell lines were derived by infection of NIH 3T3 cells with a retrovirus carrying a GFP coding sequence followed by fluorescence microscope examination to identify GFP-expressing clones. Lines were subcloned until they appeared to contain only a single location pattern. In most cases, only a single GFP-containing sequence was detectable by RT-PCR. 3D fluorescence microscope images of 46 tagged clones were taken using a 60x 1.4 NA objective on an Olympus IX50 microscope outfitted with a Nipkow disk confocal imaging system (Solamere Technology Group). The system consisted of a Yokogawa CSU10 Confocal Scanner Unit illuminated via optical fiber from a LaserPhysics Reliant 100s-488 Argon ion laser. Images were captured by a Roper Scientific/Photometrics CoolSnap HQ Cooled CCD Camera, and image capture was controlled by QED software (QED Imaging, Pittsburgh, PA, USA) on an Apple PowerMac G4 computer The pixel spacing in both directions in the image plane was 0.11 µm and the vertical spacing between adjacent planes (slices) was 0.5 µm. The resulting 1280 x 1024 x 31 3D images each contained from 1 to 3 cells (or partial cells). A total of 933 images containing 989 full cell images were obtained, with each clone having from 16 to 33 cell images. Data Set ZIP File Training Images Test Images -------- -------- --------------- ----------- -References- X. Chen, M. Velliste, S. Weinstein, J.W. Jarvik and R.F. Murphy (2003). Location proteomics - Building subcellular location trees from high resolution 3D fluorescence microscope images of randomly-tagged proteins. Proc. SPIE 4962: 298-306. -License Information- All data downloaded from the Murphy Lab web site (http://murphylab.web.cmu.edu) remain the property of the Murphy Lab. You are granted a non-exclusive license to use these images for non-commercial, research purposes, with the following conditions: (a) you agree to include a reference to the paper cited above when presenting or publishing your results, (b) you agree to send a copy of any paper or abstract (upon its acceptance for publication or presentation) by FAX, email or regular mail to Dr. Murphy, and (c) you agree not to distribute these images without inclusion of this notice. Dr. Robert F. Murphy Associate Professor of Biological Sciences Carnegie Mellon University 4400 Fifth Ave., Pittsburgh, PA 15213 FAX: 412-268-6571 email: murphy@cmu.edu