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Murphy Lab - Protocols - Flow Cytometry - Multivariate Analysis
Note
The information in this page is supplemental to the protocols found in:
- Michael V. Boland and Robert F. Murphy (1998). "Multivariate Analysis"
in Current Protocols in Cytometry. John Wiley & Sons.
Data Files
llb08.fcs |
402944 Bytes |
FCS file used for examples in the protocol. 50176 events, 4
parameters. |
llb08.txt |
852992 Bytes |
Text version of the llb08.fcs file. |
llb08s30.fcs |
243200 Bytes |
Shortened version (30,208 events) of llb08.fcs for use
with Autoklus. |
S-Plus Functions
factorial() |
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IMPORTANT: factorial() is needed by
dotplotall() and plotall2d(). Make sure to download this file and
install it before using these other functions |
dotplotall() |
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S-Plus function to plot all pairs of parameters, showing each cluster as a single color. The output is similar to Autoklus. |
plotall2d() |
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Generate contour plots for all pairs of parameters. |
plotkmeans() |
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Useful for generating contour plots where the clusters
are plotted one at a time using the same two parameters. |
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