1. R. F. Murphy (1985) Analysis and isolation of endocytic vesicles
by flow cytometry and sorting: Demonstration of three kinetically distinct
compartments involved in fluid-phase endocytosis. Proc. Natl. Acad.
Sci. U.S.A. 82:8523-8526.
- Detection of endocytic vesicle subpopulations using a fluorescent fluid-phase
marker (Figs. 1 and 2)
- Detection of endocytic vesicles using a fluorogenic substrate (Fig.
3)
- Sorting of endocytic vesicles (Fig. 4)
2. R.F. Murphy and M. Roederer (1986) Flow cytometric analysis of
endocytic pathways. In: Applications of Fluorescence in the
Biomedical Sciences, D.L. Taylor, A.S. Waggoner, R.F. Murphy, F. Lanni,
R. Birge (eds.), Alan R. Liss, Inc., New York, pp. 545-566.
- Normalization of vesicle fluorescence measurements using side scatter
(Fig. 7)
3. R.F. Murphy (1988) Processing of endocytosed material. Adv.
Cell Biol. 2:159-180.
- Two-color analysis of endocytic vesicles using two fluid-phase markers
(Fig. 4)
- Correlation of flow cytometric analysis with density gradient fraction
(and incorporation of fraction number as a "flow cytometric parameter"
to allow display of contour maps, etc.) (also in Fig. 4)
4. R.B. Wilson and R.F. Murphy (1989) Flow-cytometric analysis of
endocytic compartments. Methods Cell Biol. 31:293-317.
- Description of bead calibration for SOFA (Fig. 6)
- Detection of in vitro acidification of endocytic vesicles using acridine
orange (Fig. 7)
5. R.F. Murphy, M. Roederer, D.M. Sipe, C.C. Cain, and R. Bowser (1989)
Determination of the biochemical characteristics of cndocytic compartments
by flow cytometric and fluorometric analysis of cells and organelles.
In: Flow Cytometry: Advanced Research and Clinical Applications,
Volume II, A. Yen (ed.), CRC Press, Boca Raton, FL, pp. 221-254.
- Detection of organelles with Nile Red (combined with density gradient
fractionation) (Fig. 11)
- Detection of endocytic vesicle subpopulations using a receptor-mediated
probe (Fig. 13)
6. M.L. Zeidel, T. Hammond, B. Botelho, and H. W. Harris, Jr. (1992)
Functional and structural characterization of endosomes from toad bladder
epithelial cells. Am. J. Physiol. 263:F62-F76.
7. F.G. van der Goot, A. Seigneur, J.C. Gaucher, and P. Ripoche (1992)
Flow cytometry and sorting of amphibian bladder endocytic vesicles containing
ADH-sensitive water channels. J. Membr. Biol. 128:1330139.
8. R.M. Guasch, C. Guerri, J.E. O'Connor (1993) Flow cytometric analysis
of concanavalin A binding to isolate Golgi fractions from rat liver.
Exp. Cell Res. 207:136-141.
9. T.G. Hammond, R.R. Majewski, D.J. Morre, K. Schell, L.W. Morrissey
(1993) Forward scatter pulse width signals resolve multiple populations
of endosomes. Cytometry 14:411-420.
10. T.G. Hammond, D.J. Morre, H.W. Harris, Jr., and M.L. Zeidel (1993)
Isolation of highly purified, functional endosomes from toad urinary
bladder. Biochem. J. 295:471-476.
11. H.W. Harris, Jr., M.L. Zeidel, I. Jo, and T.G. Hammond (1994) Characterization
of purified endosomes containing the antidiuretic hormone-sensitive water
channel from rat renal papilla. J. Biol. Chem. 269:11993-12000.